Interleukin-8 (IL-8) is a proinflammatory CXC chemokine associated with the promotion of
neutrophil chemotaxis and degranulation. This chemokine activates multiple intracellular …

Proteins do not naturally lend themselves to high-throughput analysis because of their diverse
physiochemical properties. Consequently, affinity tags have become indispensable tools …

Although they are often exploited to facilitate the expression and purification of recombinant
proteins, every affinity tag, whether large or small, has the potential to interfere with the …

Although it is usually possible to achieve a favorable yield of a recombinant protein in Escherichia
coli, obtaining the protein in a soluble, biologically active form continues to be a major …

Because of its stringent sequence specificity, the catalytic domain of the nuclear inclusion
protease from tobacco etch virus (TEV) is a useful reagent for cleaving genetically engineered …

Affinity tags have become indispensable tools for protein expression and purification. Yet,
because they have the potential to interfere with structural and functional studies, it is usually …

… permission in writing from the publisher or Geophotos, 6.48 David Waugh, 6.50 J.Allan
Cash/J. Allan Cash under licence from The Copyright Licensing Agency Ltd, 90 Tottenham Court …

This chapter describes a simple method for overproducing a soluble form of the tobacco etch
virus (TEV) protease in Escherichia coli and purifying it to homogeneity so that it may be …

Background: Aquifex aeolicus Ribonuclease III (Aa-RNase III) belongs to the family of Mg 2+
-dependent endonucleases that show specificity for double-stranded RNA (dsRNA). RNase …

Because of its stringent sequence specificity, the 3C-type protease from tobacco etch virus (TEV)
is frequently used to remove affinity tags from recombinant proteins. It is unclear, …