Sulfonyl fluorides as inhibitors of esterases. I. Rates of reaction with acetylcholinesterase, α-chymotrypsin, and trypsin

DE Fahrney, AM Gold - Journal of the American Chemical Society, 1963 - ACS Publications
The second-orderrate constants for inactivation of acetylcholinesterase,-chymotrypsin, and
trypsin by a series of sulfonyl fluorides havebeen determined. These compounds are most …

Sulfonyl Fluorides as Inhibitors of Esterases. II. Formation and Reactions of Phenylmethanesulfonyl α-Chymotrypsin*

AM Gold, D Fahrney - Biochemistry, 1964 - ACS Publications
Phenylmethanesulfonyl fluoride reacts stoichiometrically with a-chymotrypsin, producing an
inactive monosulfonyl enzyme and one equivalent of free acid. Titration of the enzyme with …

Urinalysis in acute care of adults: pitfalls in testing and interpreting results

…, C Ronan, K Montazeri, K Wai, A Gold… - Open forum …, 2014 - academic.oup.com
Background. Rapid urine tests for infection (urinalysis, dipstick) have low up-front costs.
However, many false positives occur, with important downstream consequences, including …

Sulfonyl Fluorides as Inhibitors of Esterases. III. Identification of Serine as the Site of Sulfonylation in Phenylmethanesulfonyl α-Chymotrypsin*

AM Gold - Biochemistry, 1965 - ACS Publications
S-Aminoethylcysteine is also obtained when 1-dimethylaminonaphthalene-5-sulfonyl
chymotrypsin is treated as described. This indicates that the corresponding sulfonyl chloride …

Femoral artery Doppler ultrasound is more accurate than manual palpation for pulse detection in cardiac arrest

AL Cohen, T Li, LB Becker, C Owens, N Singh, A Gold… - Resuscitation, 2022 - Elsevier
Objectives Our primary objective was to assess the accuracy of Doppler ultrasound versus
manual palpation in detecting any pulse with an arterial line waveform in cardiac arrest. …

[HTML][HTML] Analysis of a 43-kDa glycoprotein from the intracellular parasitic nematode Trichinella spiralis.

…, D Despommier, DE Misek, RI Polvere, AM Gold… - Journal of Biological …, 1992 - Elsevier
The L1 larvae of the parasitic nematode Trichinella spiralis invade skeletal muscle and initiate
a process that has been interpreted to represent skeletal muscle dedifferentiation. In this …

[83] Sulfonylation with sulfonyl halides

AM Gold - Methods in enzymology, 1967 - Elsevier
Publisher Summary This chapter describes the mechanism of sulfonylation with sulfonyl
halides. Sulfonyl halides react with many of the serine esterases such as chymotrypsin, trypsin, …

Trichinella spiralis: secreted antigen of the infective L1 larva localizes to the cytoplasm and nucleoplasm of infected host cells

DD Despommier, AM Gold, SW Buck, V Capo… - Experimental …, 1990 - Elsevier
Antibodies were elicited against a purified antigen with an apparent molecular weight of 43K.
This antibody preparation also detected a second antigen consisting of a group of closely …

Clinical evaluation of a new method for the determination of serum thyroxine

…, CJ PATTEE, A GOLD - The Journal of Clinical …, 1966 - academic.oup.com
A method for the rapid determination of thyroxine (T 4 ) in serum was described in 1964 (J Clin
Endocr 24: 187) and modified in 1965 (J Lab Clin Med 66: 161). Since the intact thyroxine …

Partial characterization of two antigens secreted by L1 larvae of Trichinella spiralis

AM Gold, DD Despommier, SW Buck - Molecular and Biochemical …, 1990 - Elsevier
Two protein antigens were isolated from excretory-secretory products of Trichinella spiralis
by biochemical methods and characterized with respect to their chemical and immunological …