Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-adjacent
motif (PAM), limiting target site recognition to a subset of sequences. To remove this …
motif (PAM), limiting target site recognition to a subset of sequences. To remove this …
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
Broad use of CRISPR–Cas12a (formerly Cpf1) nucleases 1 has been hindered by the
requirement for an extended TTTV protospacer adjacent motif (PAM) 2 . To address this limitation, …
requirement for an extended TTTV protospacer adjacent motif (PAM) 2 . To address this limitation, …
Discovery of widespread type I and type V CRISPR-Cas inhibitors
Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic
elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit …
elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit …
Racial and ethnic disparities in buprenorphine treatment duration in the US
Methods| This cohort study analyzed a random sample of buprenorphine prescriptions in
IQVIA Longitudinal Prescription Data that included race and ethnicity information and were …
IQVIA Longitudinal Prescription Data that included race and ethnicity information and were …
Active testing of groups at increased risk of acquiring SARS-CoV-2 in Canada: costs and human resource needs
…, ML Bastos, Z Lan, S Law, CE Oh, WA Russell… - Cmaj, 2020 - Can Med Assoc
BACKGROUND: Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
is largely passive, which impedes epidemic control. We defined active testing strategies for …
is largely passive, which impedes epidemic control. We defined active testing strategies for …
Screening the blood supply for Zika virus in the 50 US states and Puerto Rico: a cost-effectiveness analysis
WA Russell, SL Stramer, MP Busch… - Annals of Internal …, 2019 - acpjournals.org
Background: In 2016, universal individual donation nucleic acid testing (ID-NAT) of donated
blood for Zika virus began in US states and territories. Objective: To assess the cost-…
blood for Zika virus began in US states and territories. Objective: To assess the cost-…
Dry forests in the Southern Interior of British Columbia: Historic disturbances and implications for restoration and management
W Klenner, R Walton, A Arsenault… - Forest Ecology and …, 2008 - Elsevier
We critically examine the hypothesis that dry forests in southern British Columbia evolved in
the context of a low-severity fire-dominated disturbance regime, that fire suppression has led …
the context of a low-severity fire-dominated disturbance regime, that fire suppression has led …
Impact of alternative ways to operationalize buprenorphine treatment duration on understanding continuity of care for opioid use disorder
Methods We utilized buprenorphine prescription records from the IQVIA Real World Longitudinal
Prescription Data (LRx). We analyzed a random sample, containing 867,788 patients …
Prescription Data (LRx). We analyzed a random sample, containing 867,788 patients …
Individualized risk trajectories for iron‐related adverse outcomes in repeat blood donors
Background Despite a fingerstick hemoglobin requirement and 56‐day minimum donation
interval, repeat blood donation continues to cause and exacerbate iron deficiency. Study …
interval, repeat blood donation continues to cause and exacerbate iron deficiency. Study …
[HTML][HTML] Cost-effectiveness of increasing buprenorphine treatment initiation, duration, and capacity among individuals who use opioids
Importance Buprenorphine is an effective and cost-effective medication to treat opioid use
disorder (OUD), but is not readily available to many people with OUD in the US. The current …
disorder (OUD), but is not readily available to many people with OUD in the US. The current …