A fish hepatoma cell line (PLHC-1) as a tool to study cytotoxicity and CYP1A induction properties of cellulose and wood chip extracts
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Toxicity assessment of Lemna solid waste dumpsite (Calabar, Nigeria) using different extraction methods and toxicological responses of PLHC-1 cells
2021, Environmental Toxicology and PharmacologyCitation Excerpt :Therefore, the aim of this present study was to evaluate the effects of different soil (collected from a solid waste dumpsite) extracts on PLHC-1 cells and targeting toxicological endpoints at transcriptional and functional product levels for biotransformation, antioxidant and cellular protection responses. The PLHC-1 cell line has been demonstrated as an excellent toxicological model due to their potent induction of Cyp1a activity following exposure to several environmental contaminants (Huuskonen et al., 1998; Thibaut et al., 2009; Mennillo et al., 2020). Our hypothesis is that exposure of PLHC-1 cells to soil extracts from a solid waste dumpsite will produce toxicological responses in cells and that these responses will be dependent on extraction method, exposure concentration and time, and individual parameter.
Integration of fish cell cultures in the toxicological assessment of effluents
2019, Ecotoxicology and Environmental SafetyCitation Excerpt :The hepatoma fish cell line PLHC-1 was chosen as experimental system in the TIE and mode of action studies because it has several advantages over fibroblast-like cells, such as RTG-2. PLHC-1 cells present more metabolic activity, expressing aryl hydrocarbon receptors and inducible and stable cytochrome P450 enzymes, which are involved in the metabolism of environmental chemicals (Huuskonen et al., 1998). This cell culture has a high rate of proliferation and is ideally suited to assess the acute and chronic toxicity and to derive structure-activity relationships (Fent, 2001; Zurita et al., 2007a).
Applicability of in vitro methods in evaluating the biotransformation of polycyclic aromatic hydrocarbons (PAHs) in fish: Advances and challenges
2019, Science of the Total EnvironmentXenobiotic metabolism in the fish hepatic cell lines Hepa-E1 and RTH-149, and the gill cell lines RTgill-W1 and G1B: Biomarkers of CYP450 activity and oxidative stress
2018, Comparative Biochemistry and Physiology Part - C: Toxicology and PharmacologyCitation Excerpt :However, the increased EROD activity shown by Hepa-E1 cells after exposure to both BNF and BaP, makes this cell line a suitable candidate for in vitro experimentation of xenobiotic metabolism and CYP expression. PLHC-1 has been the most used cell line to evaluate CYP450 activity (Caminada et al., 2006; Fent, 2001; Huuskonen et al., 1998). While excellent results may be obtained with it, effective alternatives must be available to expand the applicability of in vitro studies.
Aluminum smelter-derived polycyclic aromatic hydrocarbons and flatfish health in the Kitimat marine ecosystem, British Columbia, Canada
2015, Science of the Total EnvironmentCitation Excerpt :Yunker et al. (2011) also reported finding other plant-derived PAHs, including naturally occurring diterpenes, steradienes, and their derivatives in sediments and clams from Kildala Arm. Such compounds found in cellulose, wood chips, and other wood products, which could occur naturally at the Kildala Arm and Kitlope sites, are also known to induce CYP1A in fish (Martel and Kovacs, 1997; Huuskonen et al., 1998; Hewitt et al., 2006). In summary, although this study showed consistent uptake of PAHs by sole within Kitimat Arm, especially at sites nearest the smelter, exposure levels and health effects in fish were consistently lower than those that have been observed in this species at other sites contaminated with PAHs from different sources (e.g., creosote, petroleum products, vehicle emissions, and other combustion processes).
Stem Cells from Cartilaginous and Bony Fish
2008, Methods in Cell BiologyCitation Excerpt :Contributions of fish cell cultures to this discipline at a biochemical level include studies of xenobiotics and identification of the metabolites that are produced through CYP (P450)‐mediated oxidation (epoxide formation and breakdown), and gluthione or glucuronide conjugation (Bols et al., 1999; Collodi et al., 1994; Jung et al., 2001; Tom et al., 2001; Zabel et al., 1996). The aryl‐hydrocarbon receptor (AHR) has been studied in a number of fish cell types and experimental situations (Choi and Oris, 2003; Hestermann et al., 2002a,b; Huuskonen et al., 1998). In addition, cellular and molecular aspects of fish immunology critically relevant to comparative biomedicine and human health have been investigated using both primary cell cultures and immortalized cell lines (Bols et al., 2001, 2003; Clem et al., 1996).