Stability of SARS-CoV-2 in donated human milk with and without Holder pasteurization (TCID50/mL)*
| Sample | Unpasteurized (room temperature for 30 min) | Pasteurized (62.5°C for 30 min) |
|---|---|---|
| A | 2.0 × 105 | Undetected |
| B | 6.3 × 104 | Undetected |
| C | 6.3 × 105 | Undetected |
| D | 6.3 × 106 | Undetected |
| E | 6.3 × 105 | Undetected |
| F | 2.0 × 105 | Undetected |
| G | 6.3 × 105 | Undetected |
| H | 6.3 × 105 | Undetected |
| I | 6.3 × 105 | Undetected |
| J | 6.3 × 105 | Undetected |
| SARS-CoV-2 alone (positive control) | 6.3 × 106 | 6.3 × 103 |
| Mock infection (negative control) | Undetected | Undetected |
Note: SARS-CoV-2 = severe acute respiratory syndrome coronavirus 2, TCID50 = 50% of the tissue culture infectivity dose.
↵* TCID50/mL calculations defined by duplicate dilution series of indicated samples.