In this issue, Suh and colleagues report on the risk of atherothrombotic events in people taking clopidogrel.1 Their main objective was to link clopidogrel efficacy to the activity of a specific CYP3A isoenzyme, namely CYP3A5, in people with 2 different CYP3A5 genotypes. The rationale for their study is based on the fact that clopidogrel is a prodrug that requires metabolism by CYP3A4 and CYP3A5 before it can be active.2–4 Therefore, Suh and colleagues conducted their study to shed light on a broader, controversial issue: Is decreased CYP3A5 activity that is due to drug–drug interactions or genetic polymorphisms associated with a clinically significant decrease in clopidogrel efficacy?
In phase 1 of their study, Suh and colleagues enrolled 16 healthy volunteers with the CYP3A5 expressor genotype (*1/*1 or *1/*3 allele) and 16 with the CYP3A5 non-expressor genotype (*3/*3 allele). They then gave them two 7-day courses of clopidogrel; the second course was preceded by a 4-day course of itraconazole, a selective CYP3A4 inhibitor. Inhibition of CYP3A4 by itraconazole in the subjects who lacked CYP3A5 was associated with a significant decrease in clopidogrel activity, whereas some activity (about 25%) was preserved in those with the CYP3A5 expressor genotype.
In phase 2 of their study, Suh and colleagues observed the frequency of atherothrombotic events (cardiac death, myocardial infarction and nonhemorrhagic stroke) in a 6-month follow-up period among 348 patients who underwent coronary angioplasty with bare-metal stent implantation and antiplatelet therapy with clopidogrel. They found that such events occurred more frequently among the patients who had the CYP3A5 non-expressor genotype (14/193) than among those with the expressor genotype (3/155). Results from both phases of their study suggest that decreased metabolism of clopidogrel to its active form is associated with decreased clinical efficacy of the drug.
In 2003, Lau and colleagues showed that, in 44 patients undergoing percutaneous coronary intervention (PCI) with stent implantation, concomitant treatment with atorvastatin, a CYP3A substrate, was associated with reduced antiplatelet activity of clopidogrel.5 They also showed a reduction in clopidogrel's antiplatelet activity associated with the use of erythromycin and troleandomycin, 2 potent CYP3A inhibitors; however, use of rifampin, a CYP3A inducer, was associated with enhanced antiplatelet activity. To confirm these observations, Lau and colleagues conducted another study, in which they found further evidence of a relationship between CYP3A4 activity and clopidogrel activity.6 Neubauer and associates observed in 47 patients undergoing elective PCI that pretreatment with atorvastatin or simvastatin was associated with a reduction in clopidogrel effects.7 Others also observed a lower efficacy of clopidogrel in healthy volunteers taking certain statins but not others.8
It is noteworthy that the results reported by Lau and colleagues5 were questioned because of their use of nonstandard platelet function assays. As well, a number of prospective clinical trials and retrospective epidemiologic studies failed to show a clinically significant interaction between clopidogrel and statins.9–17 However, several of these studies had major limitations: they considered all statins, not only those that are CYP3A inhibitors, and they did not take into account in their control groups the concomitant intake of various CYP3A substrates (e.g., calcium-channel blockers, antidepressants, benzodiazepines, macrolide antibiotics, imidazole antifungals) that can modulate clopidogrel activity.
The study of genetic contributions to drug action, including pharmacokinetics and pharmacodynamics, may help to improve the efficacy and safety of drugs. Of the CYP enzymes, the CYP3A enzymes are the most relevant, catalyzing the biotransformation of more than 50% of currently used therapeutic drugs. Consequently, variations in CYP3A activity can affect the efficacy and safety of drugs metabolized by these isoenzymes. Many research groups have failed to show an association between CYP3A4 variants and enzyme activity. In contrast, CYP3A5 is polymorphically expressed and shows marked differences between ethnic populations.18–20 Single nucleotide polymorphisms CYP3A5*3 and CYP3A5*6 lead to alternative splicing and protein truncation, which results in the absence of CYP3A5 from tissues.19–21 When CYP3A5 is expressed (in subjects with at least one CYP3A5*1 allele), this protein may account for more than 50% of the total CYP3A activity in the liver.19
Suh and colleages used itraconazole to selectively inhibit CYP3A4 while preserving some activity in subjects with the CYP3A5*1 allele. Studies of in vitro drug metabolism have shown that only CYP3A4 catalyzes the biotransformation of itraconazole, whereas CYP3A5 exhibits no catalytic capabilities.22 Yu and associates found that subjects with the CYP3A5*1 allele were less susceptible to changes in systemic clearance of midazolam during inhibition by itraconazole than were subjects with similar total CYP3A activity but who had CYP3A5*3 alleles.23 These results suggest that itraconazole is an appropriate tool to selectively inhibit CYP3A4 activity and not CYP3A5 activity.
The active metabolite of clopidogrel irreversibly blocks P2Y12 receptors on the platelet surface, which inhibits adenosine diphosphate (ADP)-induced platelet aggregation.24,25 Hence, in addition to CYP3A5 polymorphisms, mutations in the P2Y12 gene may also account for the variability in clopidogrel efficacy. Three single-nucleotide polymorphisms and a single-nucleotide insertion polymorphism in the P2Y12 gene define 2 haplotypes: patients with a H2 haplotype have been found to exhibit increased maximal platelet aggregation in response to ADP and possibly a lesser response to drugs such as clopidogrel.26 This potential genetic variability in the pharmacodynamics of clopidogrel was not taken into account in the study by Suh and colleagues.
Platelets play an important role in the pathogenesis of atherothrombotic events. Patients undergoing PCI with stent implantation are at an increased risk of thrombosis sometimes complicated by acute myocardial infarction or death. It is estimated that more than 1 million PCI procedures are performed annually worldwide and that most (70%) involve stent implantation.27 Currently, the American Heart Association/ American College of Cardiology recommends that all patients undergoing PCI with implantation of a bare-metal stent receive dual antiplatelet therapy, with ASA and a thienopyridine, for at least 1 month after the procedure (3–6 months for drug-eluting stents), and ideally up to 12 months in patients who are not at high risk of bleeding.27
The results reported by Suh and colleagues represent significant clinical findings that clearly call for well-designed, prospective, pharmacogenetic, drug–drug interaction studies to ascertain clopidogrel's efficacy in patients with various genotypes receiving treatment with CYP3A substrates.
Besides inhibition of CYP3A, other reasons, such as impaired intestinal absorption28,29 dose-and time-dependency30 and post-treatment platelet reactivity,31 should be evaluated to gain a complete understanding of the variability observed in clopidogrel action.
@ See related article page 1715
Footnotes
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This article has been peer reviewed.
Contributors: All of the authors contributed substantially to the analysis of the data and the writing of the manuscript, and all approved the final version.
Competing interests: None declared for Chantal Pharand or Véronique Michaud. Jacques Turgeon has received speaker fees from Pfizer Canada.