Methods The technique of measuring lymphocyte re¬ sponse to phytohemagglutinin has
already been described. 1 Briefly, lymphocytes are obtained by taking blood into heparin …

METHODS AND MATERIALS Lymphocyte Culture Technique Lymphocytes were obtained
from the peripheral blood as previously described and were divided into two aliquots. 12 …

THE immune response of the body to the tubeicle bacillus is apparently accomplished by
both circulating" humoral" antibody'and by cell-mediated immune reaction. 2 The …

A method for quantitative evaluation of phytohemagglutinin (PHA) stimulated lymphocyte
cultures is presented. With 3 per cent gelatin to enhance erythrocyte sedimentation and …

Results The per cent transformation of lymphocytes in normal persons and in patients with
Hodgkin's disease is shown in Fig. 1. In this group the cells were grown in Eagle's media …

Now ell (1) was the first to demonstrate that phytohemagglutinin (PHA) caused human blood
mononuclear cells to transform into blastlike cells and divide in vitro. Various investigators …

Human peripheral blood small lymphocytes cultured in vitro have the potentiality of trans-
forming into large lymphoblastoid cells which are able to synthesize DNA and subsequently …

Abstract Eddie-Quartey, Alfred C., and Gross, Nicholas J.: Phytohemagglutinin activation of
lymphocytes. Quantitative comparison of different preparations. Am J Clin Pathol 69: 326 …

The report by Nowell in 1960 (I) that human blood leukocytes, long thought to be
nonproliferating, could, in fact, be stimulated in vitro by phytohemagglutinin (PHA) …

Phytohemagglutinin (PHA) has been shown to stimulate both mitoses and the formation of
blast-like cells in cultures of normal human leukocytes (1). The blast-like cells have been …