Local injury, pain or swelling is concerning for envenomation after a rattlesnake bite.
Monitoring for coagulopathies is required after massasauga rattlesnake bites.
Antivenom is the first-line treatment for envenomation.
Additional doses of antivenom may be needed; thus, close monitoring is required.
A healthy 25-year-old man was bitten by an eastern massasauga rattlesnake (Sistrurus catenatus; Figure 1) on his left great toe. He had been walking outside at night in open-toed sandals in the Eastern Georgian Bay region of Ontario. He developed lower leg edema, and pain and swelling around his great toe, but he delayed seeking medical treatment for 22 hours (Figure 2A).
On initial examination, the patient had a hemorrhagic bleb two puncture marks 1.5 cm apart on his great toe. A 12 cm × 6 cm hematoma was also present inferior to his left groin. Initial blood work showed an international normalized ratio of greater than 12.5 (normal range 0.9–1.1), partial thromboplastin time of greater than 180 (normal range 20–40) s and fibrinogen of less than 0.6 (normal range 5.1–11.8) μmol/L. His hemoglobin level and platelet count were within normal limits.
In accordance with the West Parry Sound Health Centre algorithm for rattlesnake bites, he received one vial of Antivipmyn, followed by an additional nine vials.1 (Test doses are given to allow monitoring for anaphylaxis.) His international normalized ratio and partial thromboplastin time 3.5 hours after administration of antivenom improved to 2.4 and 45 seconds, respectively. He was monitored in the intensive care unit and given an additional 10 vials of antivenom because of his ongoing pain, swelling and coagulopathy. This was followed by maintenance dosing with 12 vials of antivenom over 18 hours as his symptoms and coagulopathy began to improve.
The patient did not have any severe bleeding, and after 40 hours, the local swelling and erythema had improved substantially (Figure 2B). His international normalized ratio normalized to 1.1 and his partial thromboplastin time was 31 seconds (Box 1). Aside from antivenom, additional treatment (blood products or vitamin K) was not administered. He was discharged after four days of monitoring, during which time he received a total of 32 vials of Antivipmyn.
Discussion
The only venomous snake in Ontario, the massasauga rattlesnake resides mainly in the Eastern Georgian Bay area and the Bruce Peninsula (Figure 3).2 The Committee on the Status of Endangered Wildlife in Canada (COSEWIC) considers the snake to be threatened and it is protected under federal and provincial laws. Rattlesnake bites are uncommon, but potentially devastating. Envenomation can result in hemorrhage, increased vascular permeability, tissue necrosis and, in the worst-case scenario, death from shock.3 In a case reported by the Arizona Poison and Drug Information Center, a massasauga rattlesnake bit a 62-year-old man on the right thumb. That patient’s fibrinogen decreased to less than 1.47 μmol/L, but there were no other abnormalities in coagulation laboratory work. He did not have any bleeding and his fibrinogen levels normalized over two months.4
Pathophysiology
Venom-related coagulopathy can affect almost any component of the hemostatic system, and snakebites have been associated with platelet dysfunction, vessel wall breakdown, prothrombin overactivation and fibrinogen degradation.5,6 The nature of snake venom is complex, and a proteomic-based analysis of eastern massasauga venom showed the presence of metalloproteinases, L-amino acid oxidase, phospholipase A2 enzymes and serine proteases.7
Coagulopathy can recur after initial treatment with antivenom. The mechanism is not clearly understood, but it is thought to be a result of the clearance of the antivenom and a subsequent reappearance of the venom’s effects.8 Venom can also be deposited as a depot at the site of the bite, resulting in lingering, unneutralized venom.8
Management of rattlesnake bites
To prevent rattlesnake bites, it is important to wear ankle-height, closed-toed boots or shoes when hiking in areas with rattlesnakes. The massasauga has relatively small fangs, which are not likely to penetrate leather hiking boots or loose clothing.1,9
Initial first aid should focus on supporting the patient’s airway, breathing and circulation.10 Tourniquets, ice and attempts at venom removal should be avoided and, because of the possibility of swelling, accessories such as rings or watches should be removed. Tetanus boosters should be given for prophylaxis.1 Unless a wound infection is present, there is no role for antibiotics in the treatment of rattlesnake bites.9
Antibodies to snake venoms (antivenom) are the most efficacious treatment for viper envenomation (pit vipers are a family of snakes that includes rattlesnakes). Massasauga rattlesnake envenomation can be treated with Antivipmyn, a F(ab′)2 fragment produced in horses. It has a relatively long half-life of 133 (± standard deviation 52.53) h11 and reduced risk of rebound envenomation compared with other products. Recurrent coagulopathy has been reported after treatment with Fab antivenom in about one-third to one-half of patients.12,13 Antivenom is rotated through multiple facilities including municipal zoos, The Hospital for Sick Children in Toronto and hospitals near areas where massasauga rattlesnakes are known to reside. In Ontario, the West Parry Sound Health Centre acts as the provincial antivenom depot. Local poison control centres should be involved for rattlesnake envenomation, as they can provide direction regarding treatment and can facilitate the provision of antivenom.
Patients without pain, swelling or abnormalities in their laboratory work may have a “dry bite,” meaning that no venom was injected. “Dry bites” comprise about 25% of bites and do not require antivenom;10 these patients should be carefully monitored in hospital for at least 8 hours, and ideally 24 hours, as signs of envenomation can be delayed.14 Serial measurements of limb circumference should be taken and laboratory work, including a complete blood count, coagulation parameters and fibrinogen, should be measured at the initial assessment. These blood tests should be repeated before discharge from hospital to ensure there are no signs of envenomation.14 Careful attention to the development of coagulopathy should be given when local injury is present.
Antivenom should be administered to patients with signs and symptoms of envenomation. Therapy with antivenom should be titrated to the patient’s pain, swelling and laboratory values. Laboratory work including a complete blood count, electrolytes, creatinine, urea, glucose, creatine kinase, international normalized ratio, aspartate transaminase and alanine transaminase should be measured two hours after each dose of antivenom.1 Persistent pain or swelling, thrombocytopenia and coagulopathy are indications for further treatment.1 As symptoms and laboratory values improve, maintenance dosing of antivenom can be given and the frequency of bloodwork can be decreased.1
Upon discharge, patients should be counselled regarding reasons to seek medical attention. They should return to an emergency department if they have bleeding, worsening pain, swelling or signs of infection. Patients should avoid contact sports, elective surgery and dental work for two weeks after the bite.1 To assess for late coagulopathy, a follow-up visit with bloodwork should be scheduled two to three days and five to seven days after discharge.14
Acknowledgement
The authors thank Dr. Terence Fargher, who provided insight into and expertise on the use of Antivipmyn for massasauga rattlesnake bites.
Footnotes
Competing interests: Within the last 48 months, Mark Crowther has sat on advisory boards for Octapharma, Bayer, Boehringer Ingelheim, Janssen, Pfizer, LEO Pharma and Portola; participated in study steering committees, or other research-related activities for projects involving AKP America, Daiichi, Bayer and LEO Pharma; received a Career Investigator award from the Heart and Stroke Foundation of Ontario; and held the LEO Pharma Chair in Thromboembolism Research at McMaster University (income from which is used to support the salary of research employees). His institutions (McMaster University and St Joseph’s Healthcare) have received funding for research projects from the Heart and Stroke Foundation of Canada, LEO Pharma and Bayer for work in which Mark Crowther is involved. He has also received funding for preparation of educational materials and presentations from Alexion, Ortho Clinical Diagnostics, the BMS-Pfizer Alliance, LEO Pharma, AbbVie, Bayer, Celgene, Shire and CSL Behring, and participated in various medicolegal activities relating to thrombosis, anticoagulant drugs, or other aspects of hematological practice, and that these activities are bound by confidentiality arrangements. No other competing interests were declared.
This article has been peer reviewed.
The authors have obtained patient consent.
Contributors: Both authors contributed to the conception and design of the work, drafted the manuscript, revised it critically for important intellectual content, gave final approval of the version to be published and agreed to be accountable for all aspects of the work.